sphingotest® pro-NT

  • Proneurotensin (pro-NT) is a stable surrogate marker for Neurotensin5
  • Elevated fasting plasma levels of pro-NT are associated with an increased risk of Breast cancer2,3
  • For Obesity risk prediction see here
  • For Cardiovascular disease prediction see here

sphingotest® pro-ENK

  • pro-ENK is a stable surrogate marker for the unstable peptide hormones Met- and Leu-Enkephalin6
  • Low fasting plasma levels of pro-ENK are associated with an increased risk of breast cancer4

Medical Utility of pro-NT & pro-ENK

  1. Prediction of increased breast cancer risk in order to intensify diagnostic measures or to support hormone replacement therapy (HRT)
  2. Prevention of disease by keeping pro-NT levels in a “low risk range”, e.g. by adapting nutritional habits or by life-style adjustments

sphingotest® pro-NT/pro-ENK Immunoassays

  • sphingotest® pro-NT measures a stable fragment of the Neurotensin precursor Proneurotensin (pro-NT)
  • sphingotest® pro-ENK measures a stable mid-regional fragment of the Enkephalin precursor Proenkephalin (pro-ENK) as surrogate for the unstable Enkephalins
  • The sphingotest® pro-NT/pro-ENK Broschure can be downloaded here.


  1. http://www.nationalbreastcancer.org/breast-cancer-facts
  2. Melander O, Maisel AS, Almgren P, Manjer J, Belting M, Hedblad B, Engström G, Kilger U, Nilsson P, Bergmann A, Orho-Melander M. Plasma proneurotensin and incidence of diabetes, cardiovascular disease, breast cancer, and mortality. JAMA. 2012; 308(14):1469-75. PubMed PMID:23047361.
  3. Melander O, Belting M, Manjer J, Maisel AS, Hedblad B, Engström G, Nilsson P, Struck J, Hartmann O, Bergmann A, Orho-Melander M. Validation of plasma proneurotensin as a novel biomarker for the prediction of incident breast cancer. Cancer Epidemiol Biomarkers Prev. 2014; 23(8):1672-6. PubMed PMID: 24925674.
  4. Melander O, Orho-Melander M, Manjer J, Svensson T, Almgren P, Nilsson PM, Engström G, Hedblad B, Borgquist S, Hartmann O, Struck J, Bergmann A, Belting M. Stable Peptide of the Endogenous Opioid Enkephalin Precursor and Breast Cancer Risk. J Clin Oncol. 2015 Aug 20;33(24):2632-8. PubMed PMID:26169618.
  5. Ernst A, Hellmich S, Bergmann A. Proneurotensin 1-117, a stable neurotensin precursor fragment identified in human circulation. Peptides. 2006; 27(7):1787-93. PubMed PMID: 16519961.
  6. Ernst A, Köhrle J, Bergmann A. Proenkephalin A 119-159, a stable proenkephalin A precursor fragment identified in human circulation. Peptides. 2006; 27(7):1835-40. PubMed PMID:16621157.