Proneurotensin (pro-NT)

  • Elevated fasting plasma levels of pro-NT are associated with increased risk of:
    – Obesity2
    – Cardiovascular diseases3,4 Read more…
    – Breast cancer4,5 Read more…
  • Release of pro-NT is stimulated by the uptake of fatty acids
  • Risk of disease can be monitored by sphingotest® pro-NT and influenced by diet and   adjustments to lifestyle


Medical Utility

  1. Prediction of risk of obesity, as well as cardiovascular disease and breast cancer
  2. Prevention of disease by keeping pro-NT levels in a “low risk range”, e.g. by adapting nutritional habits or by life-style adjustments

sphingotest® pro-NT Immunoassay

  • sphingotest® pro-NT utilizes a stable fragment of the Neurotensin precursor molecule (Proneurotensin) that is produced in the same ratio as Neurotensin
  • The sphingotest® pro-NT Broschure can be downloaded here


  1. WHO Fact sheet: Obesity and overweight (June 2016)
  2. Li J, Song J, Zaytseva YY, Liu Y, Rychahou P, Jiang K, Starr ME, Kim JT, Harris JW, Yiannikouris FB, Katz WS, Nilsson PM, Orho-Melander M, Chen J, Zhu H, Fahrenholz T, Higashi RM, Gao T, Morris AJ, Cassis LA, Fan TW, Weiss HL, Dobner PR, Melander O, Jia J, Evers BM. An obligatory role for neurotensin in high-fat-diet-induced obesity. Nature. 2016; 533(7603):411-5. PubMed PMID: 27193687
  3. Fawad A, Schultz C, Nilsson PM, Orho-Melander M, Melander O. 1C.07: Proneurotensin independently predicts cardiovascular disease. The Malmö Preventive Project. J Hypertens. 2015; 33 Suppl 1:e11. PubMed PMID: 26102676.
  4. Melander O, Maisel AS, Almgren P, Manjer J, Belting M, Hedblad B, Engström G, Kilger U, Nilsson P, Bergmann A, Orho-Melander M. Plasma proneurotensin and incidence of diabetes, cardiovascular disease, breast cancer, and mortality. JAMA. 2012; 308(14):1469-75. PubMed PMID:23047361.
  5. Melander O, Belting M, Manjer J, Maisel AS, Hedblad B, Engström G, Nilsson P, Struck J, Hartmann O, Bergmann A, Orho-Melander M. Validation of plasma proneurotensin as a novel biomarker for the prediction of incident breast cancer. Cancer Epidemiol Biomarkers Prev. 2014; 23(8):1672-6. PubMed PMID: 24925674.